Glucose induced ApoB100/ApoAI ratio changes in cultured HepG2 cells in vitro

Backgroundː Numerous in vivo human cohort studies have suggested that the apolipoprotein B100/apolipoprotein AI (ApoB100/ApoAI) ratio might be a risk factor in coronary heart disease. The aim of this study was to measure ApoB100/ApoAI changes cell secretions by incubating HepG2 cells with various amounts glucose vitro. Methods ː HepG2 were cultured low-, normal- or high-glucose Dulbecco's Modified Eagle Medium (DMEM) (1, 4.5 and 10g/L, respectively). Levels ApoAI ApoB100 measured commercial sandwich enzyme-linked immunosorbent assay kits (cat#: H0123 H0124) from ShangHai MEIXUAN Biological Science Technology Ltd (Shanghai, China). Experiments repeated six times for each assay. Resultsː results showed positive correlations concentration increase. Conclusionsː A higher induced an undesirable change, which suggests new regulatory pathway lipoprotein catabolism provides model further mechanism study. This finding may lead novel therapeutic ways diagnosis treatment artery disease.